3N+淡水藻培地の作製法

1. Make up 6 stock solutions, 100 mL each in volume.
1
 NaNO3
7.5 g/100 mL
2
 CaCl2・2H2O
0.33 g/100 mL
3
 MgSO4・7H2O
0.75 g/100mL
4
 K2HPO4
0.75g/100mL
5
 NaCl
0.25g/100mL
6
 KH2PO4
1.75g/100mL
2. PII trace metals stock (1000 mL)
To make 1 L of stock, add trace metals in below order to 900 mL DDW. Bring to 1L volume with DDW.
H3BO3
1.140 g
FeCl3・6H2O
0.049 g
MnSO4・1H2O
0.123 g
ZnSO4・7H2O
0.022 g
CoSO4・7H2O
0.005 g
Na2EDTA
1.000 g
3. Soil extract
・土壌を採取，篩で小石を取り除く．
・113 gの土と1.5 gのCaC03を3 Lのフラスコに入れる．
・2 Lの一次蒸留水を加え，オートクレーブする．
・上澄みを濾過する．
4. To make 1 L of 3N+:
700 mL
DDW
10 mL
each stock solution
3 mL
PII trace metals stock
10-50 mL
Soil extract
Bring 1 L volume with DDW, after adjasting pH 6.8 with 1 N NaOH. Autoclave 20 min.

recipeへ戻る